METHOD FOR CULTIVATION OF LACTIC ACID BACTERIA USING AS GROWTH STIMULATOR OF DISINTEGRATED BIOMASS OF MICROALGAE CHLORELLA VULGARIS, ACCUMULATED DURING TREATMENT OF WASTE WATER Russian patent published in 2024 - IPC C12P7/56 C12N1/20 C12R1/07 

Abstract RU 2824891 C2

FIELD: biotechnology; ecology.

SUBSTANCE: disclosed is a method of producing lactic acid involving culturing lactic acid bacteria of the genus Bacillus coagulans for 4–5 days at temperature of 50°C, initial pH level 6.5–7.5, stirring intensity 70–80 rpm in a nutrient medium with the following composition: 600 g/l of syrupy and pasteurized beet molasses, 300 g/l of industrial water, 100 g/l of waste water, purified for 3–8 days by microalgae Chlorella vulgaris to content of ammonium cations 1 mg/l, phosphate of anions 4.5 mg/l, total microbial number 0.5 million cells/ml, with accumulated biomass of microalgae Chlorella vulgaris, which is disintegrated with the Protosubtilin r3x enzyme in amount of 0.004 mg/ml for 10 minutes at temperature of 50°C.

EFFECT: invention widens the range of methods for producing the end product.

1 cl, 2 dwg, 2 tbl

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RU 2 824 891 C2

Authors

Markin Ilya Vladimirovich

Gorbacheva Svetlana Nikolaevna

Zajtsev Igor Anatolevich

Dates

2024-08-16Published

2023-01-14Filed