ENZYMATIC SYNTHESIS OF L-NUCLEIC ACIDS Russian patent published in 2019 - IPC C12N15/10 C12N15/54 C12N9/12 C12P19/34 C12P21/00 

FIELD: chemistry.

SUBSTANCE: invention relates to enzymatic synthesis of L-nucleic acids in the presence of polymerase. Disclosed is a method of adding one or more L-nucleotides to 3'-end of L-nucleic acid, comprising a step of reacting one or more L-nucleotides with L-nucleic acid in the presence of a polymerase, wherein said polymerase is capable of adding one or more L-nucleotides to 3'-end of said L-nucleic acid, wherein said polymerase consists of an amino acid sequence, wherein amino acids of said amino acid sequence are D-amino acids. Also presented is the use of a polymerase, which consists of a sequence of amino acids, wherein the amino acids of said sequence of amino acids are D-amino acids, in a method for adding one or more L-nucleotides to 3'-end of L-nucleic acid.

EFFECT: invention provides the effective addition of one or more L-nucleotides to 3'-end of L-nucleic acid in the presence of a polymerase consisting of D-amino acids.

7 cl, 20 dwg, 10 tbl, 12 ex