FIELD: molecular biology, genetic engineering, biochemistry.
SUBSTANCE: invention can be used in different methods for analysis of nucleic acids associated with synthesis of complementary DNA sequences. Invention proposes the modified form of thermostable DNA polymerase preparing by replacing glutamate residue in the consensus sequence Ser-Gln-Ile-Glu-Leu-Arg-Val/Ile of the natural thermostable enzyme for other amino acid residue and selection of mutants eliciting enhanced ability for incorporation unusual substrate in reaction, preferably r-NTP (ribonucleoside triphosphates). Invention describes nucleotide sequences encoding the modified DNA polymerase, vector comprising thereof and strains E. coli transformed with indicated vectors and providing preparing recombinant forms of mutant enzymes. The proposed application of a new thermostable DNA polymerase, among them, as a component of sets and compositions in the sequencing reaction allows using new approaches in analysis of investigation data.
EFFECT: valuable biological and biochemical properties of DNA polymerase.
20 cl, 4 tbl, 6 ex
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