CONCENTRATE OF INFLUENZA VACCINE AND METHOD FOR ITS PREPARATION Russian patent published in 2019 - IPC C12N15/63 C12N5/02 A61K39/00 

FIELD: biotechnologies.

SUBSTANCE: invention relates to the biotechnology. Described is a method for preparing a concentrate of a vaccine against influenza containing HPV, where haemagglutinin, neuraminidase and M1 protein genes are obtained from strain A/California/04/2009; haemagglutinin gene is obtained from strain A/Michigan/45/2015, and neuraminidase and M1 protein genes are obtained from strain A/California/04/2009; haemagglutinin gene is obtained from strain A/Novosibirsk/01/2014, and neuraminidase and M1 protein genes are obtained from strain A/California/04/2009; haemagglutinin gene is obtained from strain A/HongKong/4801/2014, and neuraminidase and M1 protein genes are obtained from strain A/California/04/2009; haemagglutinin gene is obtained from strain B/Phuket/3073/13, and neuraminidase and M1 protein genes are obtained from strain A/California/04/2009 or haemagglutinin gene is obtained from strain B/Brisbane/60/2008, and neuraminidase and M1 protein genes are obtained from strain A/California/04/2009. What is presented is an influenza vaccine concentrate containing one of said HPV types.

EFFECT: invention enables to obtain HPV influenza virus having high haemagglutination activity; method also increases antigen output; besides, during the method implementation the viability and high productivity of the target proteins are preserved without the use of controlled expression in the HPV influenza cell-producing cells.

4 cl, 44 dwg, 15 tbl, 7 ex