FIELD: biotechnology.
SUBSTANCE: proposed is a recombinant plasmid DNA pET22b(+)-NS1-6×His for the expression of recombinant non-structural protein NS1 of the influenza A virus in Escherichia coli cells. Also proposed is a strain of Escherichia coli BL-21(DE3)-pET22b(+)-NS1 being a producer of recombinant non-structural protein NS1 of the influenza A virus and produced by transforming cells of Escherichia coli BL-21(DE3) with recombinant plasmid DNA pET22b(+)-NS1-6×His. Said strain is deposited in the Bioresource Centre of the Russian National Collection of Industrial Microorganisms (BRC RNCIM) of NRC Kurchatov Institute State Research Institute of Genetics under No. B-14117.
EFFECT: production of a storage-stable recombinant non-structural protein NS1 of influenza A usable as a target in the development of approaches to targeted antiviral therapy.
2 cl, 6 dwg
