FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular, to an application of Cas9 nuclease for creating a double-stranded break in a DNA molecule. Also disclosed is a method for modifying the sequence of genomic DNA of a unicellular or multicellular organism, including adding an effective amount of the above Cas9 nuclease into at least one cell of said organism.
EFFECT: invention is effective for increasing the versatility of available CRISPR-Cas9 systems and allows Cas9 nuclease to be used for cutting genomic or plasmid DNA in a larger number of specific sites and specific conditions.
5 cl, 11 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
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| DNA PROTEASE CUTTING AGENT BASED ON Cas9 PROTEIN FROM DEFLUVIIMONAS SP. | 2018 |
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| DNA POLYMER BASED ON Cas9 PROTEIN FROM BIOTECHNOLOGICALLY SIGNIFICANT BACTERIUM CLOSTRIDIUM CELLULOLYTICUM | 2018 |
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| DNA PROTEASE CUTTING AGENT BASED ON CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA | 2019 |
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| TOOL FOR CUTTING DOUBLE-STRANDED DNA USING CAS12D PROTEIN FROM KATANOBACTERIA AND HYBRID RNA PRODUCED BY FUSION OF GUIDE CRISPR RNA AND SCOUT RNA | 2020 |
|
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| MEANS FOR DETECTING NUCLEIC ACIDS BASED ON ScCas12a PROTEIN FROM SEDIMENTISPHAERA CYANOBACTERIORUM | 2023 |
|
RU2820345C1 |
