METHOD FOR DIAGNOSING HEPATIC FIBROSIS IN PATIENTS WITH CHRONIC VIRAL HEPATITIS C Russian patent published in 2019 - IPC G01N33/49 G01N33/50 

FIELD: medicine.

SUBSTANCE: invention refers to medicine and can be used in diagnosing hepatic fibrosis stage in patients with chronic viral hepatitis C (CVHC). According to the disclosed method, the DNA of the comet is determined by the degree of damages of peripheral blood lymphocytes, a clinical and general therapeutic biochemical blood test, using the predictive mathematical model based on the results of analyzes of the patients with CVHC, there are found coefficients of serial logistic regression with regularization, by means of which value of auxiliary parameters e_s1 and e_s2 by formulas e_s1=exp(-0.933-0.0498*Age+0.410*Erythrocytes-0.0128*AST-0.014*ALT-0.053*Globulins-0.023*%DNA in comet tail+0.0278*Hemoglobin); e_s2=exp(-6.497-0.043*Age+2.417*Erythrocytes-0.012*AST-0.014*ALT-0.053*Globulins-0.023*%DNA in comet tail+0.0.27*Hemoglobin), where exp – exponential function; age – patient's age (years); Erythrocytes – erythrocyte count; ACT – aspartate aminotransferase; ALT – alanine aminotransferase; Globulin – peripheral blood globulin content, g/l; %DNA in comet tail – content of DNA in tail of comet of peripheral blood lymphocytes in i-th patient (%). Then calculating probability values P for each stage of fibrosis by formulas

and stage of hepatic fibrosis, which corresponds to maximum probability value, is established.

EFFECT: invention provides more reliable and accurate determination of hepatic fibrosis stage and is characterized by low invasiveness and safety for patients.

1 cl, 3 tbl, 1 ex