DIAGNOSTIC TECHNIQUE FOR HEPATIC FIBROSIS ACCOMPANYING VIRAL HEPATITIS C Russian patent published in 2015 - IPC G01N33/50 

FIELD: medicine.

SUBSTANCE: diagnostic technique for the presence and stage of hepatic fibrosis accompanying chronic viral hepatitis C consists in determining the concentrations of gamma glutamine transferase, thrombocyte growth factor-β1, tissue inhibitor of metalloproteinases-1 and MMP-9/TIMP-2 complex in blood serum; the first stage involves calculating a probability of HCV-associated hepatic fibrosis (P_PHF) by formula: P_PHF=(e^y/(1+e^y))×100%, wherein y=-13.6+3.78·X₁-0.1·X₂ wherein X₁ is a serum value of the prognostic factor MMP-9/TIMP-2, X₂ is a serum value of the prognostic factor TIMP-1, e^y is an exponential function. If P_PHF>50%, the presence of HCV-associated fibrosis changes in the liver are predicted. At the second stage, a probability of the stage of the HCV-associated hepatic fibrosis (P_SHF) is specified by formula: P_SHF=(e^y/(1+e^y))×100%, wherein y=-12+0.134317·X₁+0.527778·X₂-0.05·X₃, wherein X₁ is a serum value of the prognostic factor MMP-9/TIMP-2, X₂ is a serum value of the prognostic factor TGF-β1; X₃ is a serum value of the prognostic factor of gamma glutamine transferase. If P_SHF>50%, the presence of the pre-cirrhotic stage of fibrosis and hepatic cirrhosis accompanying chronic viral hepatitis C (F3-4 stage); P_SHF<50% shows HCV-associated hepatic fibrosis of the primary stage (F0-2 stage).

EFFECT: method is easy to use, accessible for practical medical facilities, non-invasive and safe for patients, efficient and takes no time; it is effective for screening of the pre-cirrhotic stage of fibrosis and hepatic cirrhosis.

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