FIELD: biotechnology.
SUBSTANCE: invention represents a method consisting in application of specific oligonucleotide sequences with registration of results of CPR in real time using method of analysis of curves of melting (HRM). Reaction mixtures include specific primers, as well as an adapted buffer for Taq-polymerase, which increases sensitivity of the method.
EFFECT: invention makes it possible to reduce costs for molecular-genetic research, to increase its availability and accuracy of testing due to use of original oligonucleotide sequences and optimized reaction mixtures.
1 cl, 1 dwg, 2 ex
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