FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology, molecular biology and genetics. Disclosed is a method for determining a human genotype by mutation c.496A>G in 6 exon of a gene DPYD, involving real-time PCR with detection of results using a method of analyzing melting curves (HRM) and optimizing PCR using an adapted buffer for Taq-polymerase of composition Tris-HCl 67 mM, (NH4)2SO4 166 mM, tween-20 0.1 %, glycerine 1 %, pH 8.7.
EFFECT: invention provides high sensitivity and specificity of the method for determining a human genotype by mutation c.496A>G in 6 exon of a gene DPYD.
1 cl, 1 dwg, 3 ex
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