FIELD: biotechnology.
SUBSTANCE: invention relates to molecular biology and oncology and may be used for diagnostics of terminal mutations in gene RET, associated with genetic predisposition to cancer of thyroid (CT). The set of sequences of oligonucleotides has the following nucleotide composition: 5'-CATGGCCACTCCCAGTGC-3', 5'-CACAGGGACTCCTCAGCAC-3', 5'-CACCCCCACCCACAG-3',5'-GAGATGGGTGGCTTGTG-3', 5'-GGCTAGTGCTGTCAGGCC-3', 5'-CTAAGCACCCTAGACGCG-3', 5'-CAGGGATAGGGCCTGG-3', 5'-CCCCAAGAGAGCAACACC-3', TACGAGCCGTGCCT, GATGGGCTGCGCAG, CACGTGCTGGGCCT, TAGAAGCTGTACAT, CACGAGAAGTGGAG, TACGAGCTGAGCTG, GGAAATGGATGGGATTTG, CCATATGGACGGCAATTC.
EFFECT: invention enables to carry out efficient diagnostics of germinal mutations in gene RET, associated with genetic predisposition to cancer of thyroid, based on the frequency of mutations among patients in the Russian population.
4 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR HUMAN GENOTYPE DETERMINATION BY MUTATION c.496A>G IN 6 EXON OF DPYD GENE | 2019 |
|
RU2701375C1 |
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|
RU2674687C1 |
| METHOD OF ANALYZING TERMINAL MUTATIONS IN BRCA1, BRCA2, ATM AND PALB2 GENES USING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH AN OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2020 |
|
RU2729360C1 |
| METHOD OF GENETIC POLYMORPHISM ANALYSIS FOR CARRYING OUT POSTNATAL DNA-DIAGNOSTICS OF MUCOVISCIDOSIS | 2008 |
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RU2412247C2 |
| METHOD OF ANALYSIS OF SOMATIC MUTATIONS IN GENES EGFR, KRAS AND BRAF USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2014 |
|
RU2552483C1 |
| METHOD FOR HUMAN GENOTYPE DETERMINATION BY MUTATION IVS14+1GA B 14 INTRON OF DPYD GENE | 2019 |
|
RU2709710C1 |
| METHOD OF DETECTING DNAJB1-PRKACA CHIMERIC TRANSCRIPT IN CLINICAL TISSUE SAMPLES FROM PATIENTS WITH FIBROLAMELLAR LIVER CARCINOMA USING REAL-TIME POLYMERASE CHAIN REACTION | 2023 |
|
RU2807306C1 |
| METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN PI3K GENE USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDISATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2013 |
|
RU2549682C1 |
| TEST SYSTEM FOR MUTATION DETECTION IN HUMAN FUMARYLACETOACETATE HYDROLASE AND ALPHA-1-ANTITRYPSIN GENES | 2010 |
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RU2458131C1 |
