FIELD: biotechnology.
SUBSTANCE: present invention relates to bioengineering. Disclosed is a composition comprising a nucleic acid encoding a gene-editing protein, comprising a nuclease domain and a DNA-binding domain containing repeats with the amino acid sequence LTPvQVVAIAwxyzGHGG, where "v" is Q, D or E, "w" - S or N, "Xy" is HD, NG, NS, NI, NN or N, and "z" is GGKQALETVQRLLPVLCQD or GGKQALETVQRLLPVLCQA. Also disclosed is method in vitro changing the sequence of the target DNA molecule; a kit for changing the sequence of a target DNA molecule and a vector containing a nucleic acid encoding said protein.
EFFECT: gene-editing protein has an increased length of repeats compared to repeats in effector nuclease similar to a transcription activator (TALEN), which provides higher efficiency of editing genes.
41 cl, 16 dwg, 13 tbl, 68 ex
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