METHODS AND FORMULATIONS FOR CELLS TRANSFECTION Russian patent published in 2017 - IPC A61K48/00 C12N15/00 C07H21/02 

FIELD: biotechnology.

SUBSTANCE: method for cell reprogramming for a less differentiated state is described, comprising culturing of a differentiated cell in a reprogramming medium containing albumin, wherein albumin is treated by ion exchange resin or charcoal, transfecting of a cell of one or more synthetic RNA molecules, wherein the said one or more synthetic RNA molecules include at least one RNA molecule encoding one or more reprogramming factors, and wherein the transfection yields cells expressing one or more reprogramming factors; and repeating of step (b) at least twice for 5 consecutive days to obtain cells reprogrammed for a less differentiated state. A medium for cells culturing for reprogramming for a less differentiated state is described, comprising: Dulbecco modified Eagle's medium: F-12 medium (DMEM/F12), 10 mcg/ml of insulin, 5.5 mcg/ml of transferrin, 6.7 ng/ml of sodium selenite, 20 ng/ml of basic fibroblast growth factor (bFGF) and 5 mg/ml of albumin, wherein albumin is treated by ion exchange resin and/or charcoal. Also a medium for cells culturing for reprogramming for a less differentiated state is described, comprising: Dulbecco modified Eagle's medium: F-12 medium (DMEM/F12), 10 mcg/ml of insulin, 5.5 mcg/ml of transferrin, 6.7 ng/ml of sodium selenite, 20 ng/ml of basic fibroblast growth factor (bFGF) and 5 mg/ml of albumin, wherein less than 0.65% of albumin dry weight include lipids and/or less than 0.35% of albumin dry weight comprises free fatty acids.

EFFECT: invention expands the arsenal of ways to transfer cells to a less differentiated state.

28 cl, 11 dwg, 2 tbl, 29 ex