NUCLEASE Cpf1 FROM BACTERIUM RUMINOCOCCUS BROMII, DNA MOLECULE OR RNA CODING NUCLEASE, VECTOR CONTAINING SAID DNA MOLECULE, CRISPR/Cpf1 SYSTEM CONTAINING SAID NUCLEASE AND GUIDE RNA, HOST CELL FOR PRODUCING NUCLEASE Cpf1, METHOD OF PRODUCING NUCLEASE Cpf1 AND USE THEREOF Russian patent published in 2024 - IPC C12N9/22 C12N15/62 

FIELD: biotechnology.

SUBSTANCE: present invention relates to a nuclease enzyme and use of said enzyme. Invention relates to a Cpf1 nuclease enzyme from a Ruminococcus bromii bacterium and a DNA molecule coding said enzyme. Present invention also relates to a vector containing said DNA molecule, a CRISPR/Cpf1 system containing said nuclease and a guide RNA, a host cell for producing nuclease Cpf1, a method of producing nuclease Cpf1, using said nuclease Cpf1 to form a break in a DNA or RNA molecule, a method of forming a break in a DNA or RNA molecule using said nuclease, a delivery system comprising said nuclease Cpf1, a molecule or vector, a combination of delivery methods comprising said nuclease Cpf1, a DNA molecule or a vector, a method for modifying a target DNA locus using said nuclease, using said Cpf1 nuclease for genome editing or gene therapy and detecting nucleic acids.

EFFECT: invention widens the pool of available nucleases for genome editing.

21 cl, 15 dwg, 1 ex