ENZYMATIC ONE-REACTION REACTION OF POLYPEPTIDES DOUBLE CONJUGATION IN ONE STEP USING SORTASE Russian patent published in 2020 - IPC C07K16/00 

FIELD: chemistry.

SUBSTANCE: invention relates to a method for producing conjugate Fc of antibody region. Method involves simultaneous incubation of i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 20, where X can be any amino acid residue), ii) a second polypeptide comprising the amino acid sequence LPXTA (SEQ ID NO: 31, where X can be any amino acid residue), iii) a third polypeptide having two N-ends, wherein said polypeptide has oligoglycine Gm (m equals 2 (SEQ ID NO: 22), or 3 (SEQ ID NO: 23), or 4 (SEQ ID NO: 24), or 5 (SEQ ID NO: 25)) amino acid sequence at its first N-terminal and oligoalanine Am (m is equal to 2 (SEQ ID NO: 26), or 3 (SEQ ID NO: 27), or 4 (SEQ ID NO: 28), or 5 (SEQ ID NO: 29)) amino acid sequence at its second N-terminal, iv) a fourth polypeptide with a range of activity derived from Staphylococcus aureus A and having the amino acid sequence SEQ ID NO: 21, and v) a fifth polypeptide with grade activity obtained from A Streptococcus pyogenes A and having an amino acid sequence SEQ ID NO: 34. After simultaneous incubation of said polypeptides, the conjugate is isolated from the reaction mixture.

EFFECT: invention enables to carry out enzymatic conjugation of polypeptides in a single step using two grades having independent specificities.

4 cl, 9 dwg, 1 tbl, 6 ex