FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Disclosed is a method of producing a product of enzymatic conjugation of two polypeptides. Method includes incubating a first polypeptide comprising the amino acid sequence LPXTG (where X can be any amino acid residue), second polypeptide, which has on its end oligoalanine amino acid sequence Am (where m equals 3 or 4), and a third polypeptide with grade activity derived from grade A Staphylococcus aureus. Conjugate is then separated from the reaction mixture.
EFFECT: invention provides suppression of reverse reaction or hydrolysis of formed product.
5 cl, 4 dwg, 1 tbl, 5 ex
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