FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Disclosed is an isolated nucleic acid molecule which codes a protein with phytase activity. Nucleic acid molecule contains codon-optimizing mutations taking into account the host cell body specificity, which is Pichia pastoris. Invention also relates to an expression construct and to an expression vector comprising said isolated nucleic acid molecules, as well as a method of producing a protein having phytase enzymatic activity.
EFFECT: invention provides enhanced expression of modified phytase as compared to wild type phytase and can be used for making additives in animal fodder.
13 cl, 12 dwg, 1 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| VERSION PHYTASE FERMENTS | 2005 |
|
RU2421520C2 |
| NEW THERMOSTABLE PHYTASES WITH HIGH CATALYTIC EFFICIENCY | 2018 |
|
RU2758271C2 |
| MUTANT OF PHOSPHOLIPASE WITH HIGH ENZYMATIC ACTIVITY | 2019 |
|
RU2818353C2 |
| MUTANT RECOMBINANT THERMALLY STABLE PHYTASE (VERSIONS), DNA FRAGMENT CODING SAID PHYTASE (VERSIONS) Pichia pastoris STRAIN - PRODUCER OF SAID PHYTASE (VERSIONS) | 2009 |
|
RU2472855C2 |
| CODON-OPTIMIZED NUCLEIC ACID WHICH ENCODES SMN1 PROTEIN, AND USE THEREOF | 2020 |
|
RU2742837C1 |
| NUCLEIC ACID MOLECULE ENCODING HPV 31 L1, EXPRESSION VECTOR, HOST CELL, VIRUS-LIKE PARTICLE AND METHOD OF ITS OBTAINMENT, VACCINE, PHARMACEUTICAL COMPOSITION AND METHODS INVOLVING THEM | 2004 |
|
RU2356943C2 |
| CODON-OPTIMIZED NUCLEIC ACID THAT ENCODES B-DOMAIN-DELETED FACTOR VIII PROTEIN AND ITS USE | 2022 |
|
RU2808564C2 |
| METHOD FOR PRODUCING PROTEIN | 2006 |
|
RU2435863C2 |
| OPTIMISED EXPRESSION LI HPV45 IN YEAST | 2004 |
|
RU2360001C2 |
| PICHIA PASTORIS YEAST TRANSFORMER PRODUCING PHYTASE | 2019 |
|
RU2708446C1 |
