FIELD: biotechnology.
SUBSTANCE: disclosed is a modified polypeptide for producing fructooligosaccharides, in which the polypeptide is an Aspergillus japonicus fructosyltransferase containing the amino acid sequence SEQIDNO:1 fused with a signal peptide selected from a group comprising: Alpha factor S. cerevisiae (FAK), Alpha factor complete S. cerevisiae (FAKS), Alpha factor_T S. cerevisiae (AT), Alpha-amylase Aspergillus niger (AA), glucoamylase Aspergillus awamori (GA), inulinase Kluyveromyces maxianus (IN), invertase S. cerevisiae (IV), killer protein S. cerevisiae (KP), lysozyme Gallus gallus (LZ) and serum albumin Homo sapiens (SA), wherein the signal peptide comprises a portion of four LEKR amino acids. Also disclosed is a nucleic acid coding said polypeptide, an expression vector, a host cell and use of said polypeptide in producing fructooligosaccharides.
EFFECT: invention provides higher yield of Aspergillus japonicus fructosyltransferase.
10 cl, 8 dwg, 13 tbl, 8 ex
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