FIELD: biotechnology.
SUBSTANCE: disclosed is a modified polypeptide for producing fructooligosaccharides, in which the polypeptide is β-fructofuranosidase Aspergillus niger containing the amino acid sequence SEQ ID NO: 1, fused with a signal peptide selected from a group, including S. cerevisiae alpha-factor (FAK), S. cerevisiae alpha-factor (FAKS), S. cerevisiae alpha-factor_T (AT), Aspergillus niger alpha-amylase (AA), Aspergillus awamori glucoamylase (GA), Inulinase Kluyveromyces maxianus (IN), Invertase S. cerevisiae (IV), killer protein S. cerevisiae (KP), lysozyme Gallus gallus (LZ) and serum albumin Homo sapiens (SA), wherein the signal peptide comprises a portion of four LEKR amino acids. Also disclosed is a modified nucleic acid coding said polypeptide, an expression vector, host cell and use of said polypeptide in producing fructooligosaccharides.
EFFECT: invention provides high output of recombinant β-fructofuranosidase Aspergillus niger.
11 cl, 9 dwg, 13 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
| NUCLEIC ACIDS, VECTORS, HOST CELLS AND METHODS FOR PRODUCING FRUCTOSYLTRANSFERASE FROM ASPERGILLUS JAPONICUS | 2020 |
|
RU2837819C1 |
| MUTANT OF PHOSPHOLIPASE WITH HIGH ENZYMATIC ACTIVITY | 2019 |
|
RU2818353C2 |
| METHOD FOR MICROBIOLOGICAL SYNTHESIS OF BOVINE PROCHYMOSIN USING RECOMBINANT STRAIN PICHIA PASTORIS CONTAINING SYNTHETIC GENE OF PREPROCHYMOSIN VARIANT WITH MODIFIED SIGNALING SECRETION SEQUENCE | 2020 |
|
RU2779307C2 |
| RECOMBINANT SECRETED THERMOSTABLE BETA-GLUCANASE, RECOMBINANT STRAIN OF YEAST PICHIA PASTORIS - PRODUCER OF THIS BETA-GLUCANASE, METHOD OF MICROBIOLOGICAL SYNTHESIS OF BETA-GLUCANASE BASED ON THIS STRAIN | 2020 |
|
RU2756331C1 |
| METHODS OF IMPROVING PLANTS' HEALTH WITH APPLICATION OF FREE ENZYMES AND MICROORGANISMS EXPRESSING ENZYMES AT ELEVATED LEVEL | 2017 |
|
RU2802848C2 |
| T7 RNA POLYMERASE VARIANTS | 2018 |
|
RU2796061C2 |
| NEW THERMOSTABLE PHYTASES WITH HIGH CATALYTIC EFFICIENCY | 2018 |
|
RU2758271C2 |
| COMPOSITIONS CONTAINING BACILLUS RECOMBINANT CELLS AND ANOTHER BIOLOGICAL CONTROL AGENT | 2015 |
|
RU2736827C2 |
| RECOMBINANT PRODUCTION OF STEVIOL GLYCOSIDES | 2014 |
|
RU2741103C2 |
| RECOMBINANT CELLS PRODUCING HYALURONIC ACID | 2022 |
|
RU2839983C2 |
