FIELD: biotechnology.
SUBSTANCE: invention relates to recombinant polypeptides with phospholipase C activity, and can be used for degumming oils and fats. Disclosed is phospholipase C mutant polypeptide, amino acid sequence of which is at least 95% identical to SEQ ID NO: 4 and is characterized by the presence of proline, valine, serine and serine in positions of amino acid residues, corresponding to positions 6, 8, 10 and 104 in SEQ ID NO: 4 respectively.
EFFECT: invention provides obtaining mutant phospholipase C with high enzymatic activity, which improves efficiency of degumming and increases output of diacylglycerol (DAG) during degumming.
12 cl, 3 dwg, 1 tbl, 4 ex
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