FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology and molecular biology. Disclosed is a method for cell-free DNA synthesis involving contacting a DNA template with at least one polymerase functioning on a chain substitution principle, in the presence of nucleotides and cations of one or more metals to form a reaction mixture, where isothermal amplification of the DNA matrix takes place through replication with chain substitution, wherein additional nucleotides are fed into the reaction mixture continuously or at intervals during DNA synthesis and additionally fed metal cations, such that the ratio of metal cations: nucleotides in the reaction mixture is maintained between 9:1 and 1:9.
EFFECT: method enables improving DNA output and rate of synthesis thereof, which can be used in medicine and biotechnology.
26 cl, 13 dwg, 7 tbl, 5 ex
| Title |
Year |
Author |
Number |
| MODIFIED THERMOSTABLE DNA POLYMERASE, METHOD FOR IT PREPARING AND APPLICATION |
1997 |
- Gelfend Dejvid Khehrrou
- Kehlmehn Liza Vivian
- Rajkhert Fred Lorens
|
RU2235773C2 |
| COMPOSITIONS, METHODS AND SETS FOR SYNTHESIZING AND DETECTING NUCLEIC ACIDS |
2017 |
- Le, Ferre
- Ramamurti, Kalpit
- Uajld, Dzhojs
- Fantin, Nikol
- Leng, Dzhordan
- Dyupon, Devid
- Stivens, Dzhanko
|
RU2757416C2 |
| KIT FOR SYNTHESIS REACTION MIXTURE SYNTHESIS 3′-O-PROPARGYL-MODIFIED NUCLEIC ACID |
2014 |
|
RU2688435C2 |
| RECOMBINANT THERMOSTABLE DNA POLYMERASE, METHOD FOR ITS PREPARING AND APPLICATION |
1998 |
- Gelfand Dehvid Kh.
- Kehlmehn Liza Viv'En
- Majers Tomas V.
- Rajchert Frehd Lourens
- Sigua Kristofer Lim
|
RU2238978C2 |
| THERMOSTABLE DNA-POLYMERASE, METHOD OF ITS PREPARING, FRAGMENT OF ISOLATED DNA, METHOD OF DNA AMPLIFICATION, METHOD OF LABEL INCORPORATION IN DNA AND METHOD OF REVERSE TRANSCRIPTION |
1997 |
- Ankenbauer Val'Traud
- Markau Ursula
- Svetlichnyj Vitalij
- Shmitts-Agkheguian Gudrun
- Rajzer Astrid
- Angerer Bernkhard
- Ehbenbikhler Kristine
- Laueh Frank
- Bonch-Osmolovskaja Elizaveta
|
RU2197524C2 |
| ANALYSIS AND APPLICATION OF POLYMORPHOUS FOR ESTIMATING RISK OF CARDIOVASCULAR DISEASES |
2004 |
- Kotsian Detlef
- Chekh Jorg
- Zigler Karl-Ehrnst
- Delez Zhan-Fransua
- Rikar Sil'Ven
- Mas Sandrin
|
RU2380422C2 |
| REAGENT AND METHOD OF PREVENTING AT LEAST ONE FALSE START IN POLYMERASE CHAIN REACTION AMPLIFICATION, SETS OF REAGENTS AND OLIGONUCLEOTIDES FOR AMPLIFICATION BY POLYMERASE CHAIN REACTION |
2005 |
- Pirs Kennet
- Rajs Dzhon
- Rejs Artur
- Sanches Akiles Dzh.
- Solk Dzhess
- Uong Lorens Dzh.
|
RU2495046C2 |
| METHOD FOR AMPLIFICATION OF MATRIX NUCLEIC ACID |
2001 |
- Makkarti Tomas Valentin
- Kollinz Ruajri
|
RU2284357C2 |
| METHOD FOR HOMOGENOUS DETECTION OF ONE-STRAND AMPLIFICATION PRODUCT |
2005 |
- Pirs Kennet
- Rajs Dzhon
- Rejs Artur
- Sanches Akiles Dzh.
- Solk Dzhess
- Uong Lorens Dzh.
|
RU2460804C2 |
| METHOD OF REMOVING NUCLEIC ACID CONTAMINATION IN REVERSE TRANSCRIPTION AND AMPLIFICATION REACTIONS |
2010 |
- Elde Morten
- Lanes Olav
- Gjellesvik Dag Rune
|
RU2550277C2 |
