METHOD FOR IDENTIFICATION IN IMMUNE REPERTOIRE OF CDR3 SITES ASSOCIATED WITH DISEASES Russian patent published in 2020 - IPC C12Q1/04 C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention refers to biotechnology and molecular biology. Disclosed is a method for diagnosing a disease which causes an immune response involving sampling a tissue or blood sample in each of the plurality of subjects in a group of patients and in a control group, where the group of patients includes subjects who have the same disease, and the control group includes subjects who are healthy; lymphocyte isolation from each sample; isolating polynucleotides from lymphocytes of each sample; amplification of recovered polynucleotides using an arm-PCR method and sequencing the immune repertoire of each subject in each of the two groups to identify each unique CDR3 sequence present in the samples, and to determine the frequency of each unique CDR3 sequence; identifying sequences of CDR3, which are common between at least two subjects in each of the control group and a group of patients; ranking CDR3 sequences by frequency of frequency; identification of ligaments from each group; and identification of ligaments, which are statistically significant associated with a group of patients, with providing a disease signature.

EFFECT: method enables improving the immune response assessment, which can be used to develop diagnostic analysis for various diseases.

8 cl, 6 dwg, 1 ex