OBTAINING ANTIGEN-SPECIFIC T-CELLS Russian patent published in 2021 - IPC A61K39/39 A61K35/17 C07K14/725 A61P35/00 

FIELD: immunology.

SUBSTANCE: invention relates to the production of antigen-specific T-cells. A method for obtaining a population of antigen specific cytotoxic T-lymphocytes (CTL) having a central memory and effector memory phenotype comprises provision of a sample containing naive T-cells from a patient or a suitable donor; enrichment of the sample with CD8 + T-cells; bringing the sample into contact with paramagnetic nanoparticles that contain the following on their surface: (1) antigen-presenting complex of Сlass I MHC -peptide obtained by passive loading of nanoparticles conjugated with Сlass I MHC; (2) anti-CD28 co-stimulatory ligand, which are in the same or different populations of nanoparticles; activation of antigen-specific T-cells by placing a magnetic field near paramagnetic nanoparticles for 5 minutes to 2 hours, enrichment with antigen-specific CD8 + T-cells by extracting antigen-specific T-cells associated with paramagnetic particles using a magnetic column, and propagation of the recovered CD8 + T-cells in culture for at least 5 days to obtain a population of cells having at least 106 antigen-specific CTLs having the indicated phenotypes.

EFFECT: invention secures rapid generation of a large number of antigen-specific T-cells.

16 cl, 16 dwg, 1 ex