FIELD: biotechnology.
SUBSTANCE: invention relates to an expression vector for recombinant expression of a polypeptide of interest, comprising an expression cassette comprising a polynucleotide, coding a mutant folate receptor as a selective marker, wherein the mutant folate receptor has reduced affinity of folate binding as compared to a folate receptor of wild type.
EFFECT: derived vector enables selection of mammalian host cells able to express the polypeptide of interest with the desired output.
19 cl, 5 dwg, 14 tbl, 8 ex
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