Fc-ANTIBODY FRAGMENT HAVING HIGH BINDING AFFINITY TO FcγRIIB Russian patent published in 2025 - IPC C07K16/00 C07K16/28 C07K14/705 A61K39/395 A61P35/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, in particular to a mutant polypeptide Fc-fragment having properties of high binding affinity to FcgRIIB and having an amino acid sequence corresponding to residues from position 233 to position 332 of the Fc region of human IgG2 in accordance with EU numbering, as well as to a mutant Fc region of immunoglobulin, having properties of high binding affinity to FcgRIIB and having an amino acid sequence from position 231 to the carboxyl end of heavy chains of human IgG2 in accordance with EU numbering. Also disclosed is the use of a mutant polypeptide Fc-fragment or mutant Fc-region of immunoglobulin for constructing agonist activity of antibodies or fused proteins, an isolated polynucleotide, an expression vector, host cell, pharmaceutical composition, methods of producing mutant polypeptide Fc-fragment and mutant Fc-region of immunoglobulin and the use of the mutant polypeptide Fc-fragment and the mutant Fc-region of the immunoglobulin in the manufacture of a pharmaceutical composition having high agonist activity of the antibody or fusion protein.

EFFECT: invention has important application in optimization of antibody agonist activity.

13 cl, 17 dwg, 14 tbl, 11 ex