METHOD, COMPOSITION AND KIT FOR STABILIZING DEOXYRIBONUCLEIC ACIDS IN BIOLOGICAL SAMPLES Russian patent published in 2020 - IPC C12Q1/6806 C12N15/10 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to stabilization of human deoxyribonucleic acid (DNA) and microorganisms present in complex biological samples, such as faeces. Disclosed are a method, an aqueous composition and a kit for stabilizing DNA contained in faeces or a sample contaminated with faeces, at ambient temperature. Said biological sample is brought into contact with an aqueous composition containing a chelating agent selected from ethylene diamine tetraacetic acid (EDTA) or 1,2-cyclohexane diamine tetraacetic acid (CDTA), in a concentration of approximately 150 mM to approximately 600 mM and having pH of 9.5 to 11.5. Obtained mixture is homogenised and stored at ambient temperature.

EFFECT: group of inventions provides fast and effective DNA capture and stabilization and "instantaneous fixation" of profiles of total DNA in biological samples at ambient temperature for long periods of time.

50 cl, 29 dwg, 11 tbl, 12 ex