SET OF SYNTHETIC OLIGONUCLEOTIDES FOR DETECTING OF CORONAVIRUS RNA Russian patent published in 2020 - IPC C12Q1/6806 

FIELD: medicine; biology.

SUBSTANCE: invention refers to medicine and biology, namely to detection of SARS-CoV-2 coronavirus RNA in samples of biological material of human and animals, as well as in samples of environmental objects. Described is a set of synthetic oligonucleotides for detecting of SARS-CoV-2 coronavirus RNA includes a pair of primers selected from: 5'-GGTAAGAGTCATTTTGCTATTGGCC-3' and 5'-CTTGTAAAGTTGCCACATTCCTACG-3'; 5'-GTAAGAGTCATTTTGCTATTGGCC-3' and 5'-TTGTAAAGTTGCCACATTCCTACG-3'; 5'-TAAGAGTCATTTTGCTATTGGCC-3' and 5'-TGTAAAGTTGCCACATTCCTACG-3'; 5'-AAGAGTCATTTTGCTATTGGCC-3' and 5'-GTAAAGTTGCCACATTCCTACG-3'; 5'-AGAGTCATTTTGCTATTGGCC-3' and 5'-TAAAGTTGCCACATTCCTACG-3'; 5'-TGAGTGAAATGGTCATGTGTGG-3' and 5'-AGACCTTGAGATGCATAAGTGC-3'. From a pair of selected primers, one oligonucleotide can have a fluorescent label. A set of synthetic oligonucleotides for detecting of SARS-CoV-2 coronavirus RNA can additionally contain a fluorescent labeled oligonucleotide probe complementary or partially complementary to a nucleotide sequence flanked by a selected pair of primers.

EFFECT: technical result of the invention consists in providing universal information value of detecting of SARS-CoV-2 RNA based on RT-PCR by eliminating the risk of obtaining false-negative results of RT-PCR in the presence of mutations in the amplified region of the SARS-CoV-2 genome.

3 cl, 7 ex