FIELD: medicine; biology.
SUBSTANCE: set of synthetic oligonucleotides for the detection of RNA of the coronavirus SARS-CoV-2 is described, which includes pairs of primers: 5´-CCGGGTGTTCTTTATCAGGA-3´ and 5´-GAGGGAAAACTTCTTGGGTG-3´; 5´-ACGTCTATCAGTTACGTGCC-3´ and 5´-CCCCAAAATCAGCGAAATGC-3´´. From a pair of selected primers, one oligonucleotide may have a fluorescent mark. A set of synthetic oligonucleotides for detecting RNA of the coronavirus SARS-CoV-2 may additionally contain a fluorescently marked oligonucleotide probe, complementary or partially complementary to the nucleotide sequence flanked by a selected pair of primers.
EFFECT: detection of SARS-CoV-2 based on the multiplex reverse transcription polymerase chain reaction (RT-PCR), which allows evaluating in combination the sections of the viral genome that determine the infectivity and degree of replication of SARS-CoV-2.
3 cl, 6 ex
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