FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to a technology for producing live fluorescent microorganisms. Disclosed is a strain-producer of fluorescent protein Katushka2S based on Escherichia coli DH5α strain, transformed with expression vector plasmid pKatushka2S-B, containing nucleotide sequences of an inducible T5 promoter in the lac operon, the Katushka2S fluorescent protein fluorescent gene, the ampicillin resistance blat gene, providing intracellular production of long-red fluorescent protein Katushka2S, for performing ultra-high-performance screening.
EFFECT: invention can be used to search for antimicrobial preparations possessing bacteriostatic or bactericidal action in relation to various Escherichia coli bacterial strains.
1 cl, 3 dwg, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| BIOSENSOR BASED ON STAPHYLOCOCCUS AUREUS CELLS STABLY PRODUCING GREEN FLUORESCENT PROTEIN FOR PERFORMING ULTRAHIGH-PRODUCTIVITY SCREENING | 2017 |
|
RU2722627C2 |
| RECOMBINANT PLASMID pDUALREP2 DNA AND STRAIN TRANSFORMED THEREBY FOR DETECTION OF SUBSTANCES AND MIXTURES, INHIBITING PROTEINS BIOSYNTHESIS AND/OR SOS-RESPONSE INDUCTION IN BACTERIA | 2016 |
|
RU2620074C1 |
| BACTERIAL STRAIN ESCHERICHIA coli/pTdcR-turboYFP, HAVING SENSITIVITY TO TERAHERTZ RADIATION | 2018 |
|
RU2691308C1 |
| RECOMBINANT PLASMID DNA PTRCTE-OPH AND PRODUCER OF ENZYME ORGANOPHOSPHATE HYDROLASE | 2002 |
|
RU2232807C1 |
| SET OF RECOMBINANT FLUORESCENT STRAINS OF YERSINIA PESTIS BACTERIA OF MAIN SUBSPECIES BIOVAR AND THE ALTAI BIOVAR OF THE CENTRAL ASIATIC SUBSPECIES FOR INDICATION OF PLAGUE AGENT IN EXPERIMENTAL SAMPLES | 2020 |
|
RU2769790C1 |
| METHOD FOR DETECTION OF COPPER IONS IN THE ENVIRONMENT AND BIOSENSOR FOR ITS IMPLEMENTATION | 2015 |
|
RU2628704C2 |
| STRAIN OF ESCHERICHIA COLI BACTERIA FOR CHECKING PRESENCE OF PHENOL AND HYDROPEOXIDE IN MEDIUM | 2007 |
|
RU2348687C1 |
| PEPTINOGLYCANE HYDROLASE, EXPRESSION PLASMID, CONTAINING PEPTIDOGLYCANE HYDROLASE-CODING DNA FRAGMENT, BACTERIUM-PRODUCER AND METHOD OF MICROBIOLOGICAL SYNTHESIS OF PEPTIDOGLYCANE HYDROLASE | 2012 |
|
RU2547584C2 |
| PLASMID VECTOR pET-His8-TrxL-Acip1 STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pET-His8-TrxL-Acip1 FOR EXPRESSION OF ANTIMICROBIAL PEPTIDE ACIPENSINA-1 AND METHOD OF PRODUCING SAID PEPTIDE | 2015 |
|
RU2580031C2 |
| NUCLEIC ACID ENCODING FRET-BASED FAR-RED BIOSENSOR FOR INTRACELLULAR CASPASE 3 ACTIVITY MEASUREMENT | 2013 |
|
RU2535981C1 |
