FIELD: biotechnology.
SUBSTANCE: invention represents a method involving culturing Caco2 cells on a semipermeable membrane insert in conditions of continuous current of the culture medium in a closed cycle for 7 days at the following parameters: pressure in the circuit from minus 12±25 % kPa to 12±25 % kPa, frequency of change of pressure from 4 to 5 Hz, difference of flow rate of culture medium from 0 to 0.9 mcl/s, average rate of culture medium current 5 mcl/min.
EFFECT: invention enables to obtain a differentiated monolayer of Caco2 cells with a physiological ratio of the expression of ACE2 and TMPRSS2 genes and can be used to produce a cell model based on differentiated human colon epithelial cells line Caco2 for studying SARS-CoV-2 virus.
1 cl, 2 ex
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