METHOD FOR ASSESSING CYTOTOXICITY OF RIBOSOME-INACTIVATING PROTEINS OF TYPE II IN VITRO Russian patent published in 2016 - IPC C12Q1/68 

FIELD: toxicology.

SUBSTANCE: to increase selectivity of the method and reduced time for its implementation cultivation of cells in test culture is 15 and more minutes, pull lysates of cells of test and control of cell cultures, RNA is recovered, determining total content of ribosomal 28S RNA and content of modified fragments of 28S ribosomal RNA by RT PCR, wherein total content of 28S ribosomal RNA is determined using oligonucleotides corresponding to forward primer to distal fragment 28S r-RNA SEQ1 and reverse primer to the distal fragment 28S r-RNA SEQ2 respectively; content of modified 28S ribosomal RNA is determined using oligonucleotides corresponding to forward primer to modified fragment 28S r-RNA SEQ3, and reverse primer to modified fragment 28S r-RNA SEQ4; If content of modified 28S ribosomal RNA in test culture in comparison with control, cytotoxicity of ribosome-inactivating proteins II type; determining total number 28S ribosomal RNA and number of modified 28S ribosomal RNA in test cell culture, and fraction of modified 28S ribosomal RNA in percentage level cytotoxicity.

EFFECT: invention can be used to determine in samples of plants of toxic substances, namely ribosomal-inactivating proteins II type.

7 cl, 3 dwg, 1 tbl