POLYVALENT VECTOR VACCINE HVT Russian patent published in 2024 - IPC A61K39/12 A61K39/145 A61K39/17 A61K39/245 A61P31/12 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and virology. Disclosed is a recombinant herpes virus of turkeys (rHVT) for producing a vaccine for preventing or reducing infection with MDV, IBDV, NDV and/or AIV or associated disease signs in poultry, expressing the infectious bursitis virus (IBDV) viral protein 2 (VP2) gene and the Newcastle disease virus (NDV) fusion protein (F) gene from the first and second expression cassettes. First and second expression cassettes are inserted into a unique small (Us) region of the genome of said rHVT, both being inserted into the Us2 gene. Said rHVT also expresses the haemagglutinin (HA) gene of the avian influenza virus (AIV) from the third expression cassette, which is inserted into the unique long (UL) region of the genome of said rHVT either between the UL40 and UL41 genes, or between the UL44 and UL45 genes. Disclosed is a host bird cage for producing a vaccine; vaccine for preventing or reducing infection with MDV, IBDV, NDV and/or AIV or associated signs of disease in poultry. Disclosed is a method for producing a vaccine for poultry; use of rHVT, host cells or any combination thereof to produce a vaccine for poultry; use of the vaccine; method of preventing or reducing MDV, IBDV NDV and/or AIV infection or associated disease signs; method for vaccination of poultry.

EFFECT: vector construct provides good replication of the vector and its insertions both in vitro and in vivo, as well as effective expression of all heterologous genes at a sufficiently high level and for a considerable period of time to induce and maintain a protective immune response in the vaccinated target against all putative pathogens.

15 cl, 13 tbl, 3 ex