METHOD FOR TARGETED COVALENT IMMOBILISATION OF PROTEINS ON SURFACE OF WORKING ELECTRODE Russian patent published in 2020 - IPC G01N33/543 

Abstract RU 2733935 C2

FIELD: medicine.

SUBSTANCE: invention relates to medicine. Disclosed is a method for targeted covalent immobilisation of proteins on the surface of a carbon-containing working electrode, characterized by immersing the electrode in an electrochemical cell containing 25 % vinylbenzyl azide in dimethylformamide, applying six cyclic sweeps of the potential in the range of −0.5 V to −3 V to form a polyvinylbenzylazide film on the electrode, electrochemical deposition of Cu0 particles into a polyvinylbenzyl azide film from a copper (II) solution and incubating the electrode in an aqueous protein suspension for 30 minutes.

EFFECT: invention provides reduced duration of immobilization procedure.

3 cl, 2 ex, 2 dwg

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RU 2 733 935 C2

Authors

Svalova Tatyana Sergeevna

Medvedeva Margarita Vadimovna

Malysheva Natalya Nikolaevna

Sajgushkina Anna Aleksandrovna

Kozitsina Alisa Nikolaevna

Dates

2020-10-08Published

2019-03-26Filed