FIELD: medicine.
SUBSTANCE: invention relates to medicine. Disclosed is a method for targeted covalent immobilisation of proteins on the surface of a carbon-containing working electrode, characterized by immersing the electrode in an electrochemical cell containing 25 % vinylbenzyl azide in dimethylformamide, applying six cyclic sweeps of the potential in the range of −0.5 V to −3 V to form a polyvinylbenzylazide film on the electrode, electrochemical deposition of Cu0 particles into a polyvinylbenzyl azide film from a copper (II) solution and incubating the electrode in an aqueous protein suspension for 30 minutes.
EFFECT: invention provides reduced duration of immobilization procedure.
3 cl, 2 ex, 2 dwg
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Authors
Dates
2020-10-08—Published
2019-03-26—Filed