METHOD FOR IMMOBILIZING SHORT NUCLEOTIDE SEQUENCES ON THE SURFACE AND END REGIONS OF NANOMATERIALS Russian patent published in 2021 - IPC B82Y5/00 C07H21/02 G01N27/49 B05D1/18 

FIELD: biology.

SUBSTANCE: invention relates to a method for immobilizing short nucleotide sequences of aminomodified DNA or RNA oligonucleotides on the surface and end regions of nanomaterials and can be used in industry in the production of biocomposites. Disclosed is a method for immobilizing short nucleotide sequences of aminomodified DNA or RNA oligonucleotides on the surface and end regions of nanomaterials containing oxygen-containing functional groups, including preparing a solution of oligonucleotides with a concentration of at least 50 mcM in a buffer solution with a pH in the range of 4-6.9, mixing the specified solution with a solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide with a concentration of at least 5 mM, in a buffer based on 2-(N-morpholino)ethanesulfonic acid (MES) and ethanol and applying the final solution to a substrate in the sensitive area of ​​the formed biosensor followed by keeping the solution for at least 12 hours on the substrate in order to maximize the complete reaction of binding DNA or RNA oligonucleotides with carbon nanomaterial containing oxygen-containing functional groups.

EFFECT: disclosed is a new effective method that makes it possible to form biological sensors based on carbon nanomaterials.

6 cl, 2 dwg, 1 ex