FLOW CELL SUBSTRATE STRUCTURING SYSTEM AND METHOD Russian patent published in 2024 - IPC B01J19/00 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to methods for structuring flow cell substrates (embodiments), a flow cell for a photoinitiated chemical reaction, a method for sequencing nucleic acids and a system for photoinitiated chemical reactions. Method for structuring flow cell substrates using photoinitiated chemical reactions includes making a planar waveguide flow cell by forming a layer of radiation input gratings on a glass substrate; applying a middle layer on a layer of radiation input gratings; applying a coating layer on the middle layer; and forming nanowells in coating layer; silanising the coating layer; coating the silanised coating layer and nanowells with the first group of reagents; introduction of the second group of reagents into nanowells. Second group of reagents includes a target reagent and a photosensitive photoinitiator system. According to the method, a light source is connected to the radiation input gratings and the light is directed inside a planar waveguide flow cell for photoinitiation of a chemical reaction between the first and second groups of reagents. Photoinitiated chemical reaction covalently binds the target reagent only to the lower part of each nanowell.

EFFECT: group of inventions provides elimination of excessive polishing, insufficient polishing and scratching or damage of structured surfaces during preparation of intermediate areas between nanowells.

44 cl, 6 dwg