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2 735 281

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IPC

C07K14/705(2006-01-01)

C12N15/62(2006-01-01)

C12N15/63(2006-01-01)

(21) (22)

Application

2019137972, 2019-11-25

(24)

Start date

2019-11-25

(22)

Actual filing date

2019-11-25

(45)

Published

2020-10-29

(72)

Inventor

Safronova Nadezhda AleksandrovnaLuginina Aleksandra PavlovnaBorshchevskij Valentin IvanovichGusach Anastasiya YurevnaMarin Egor VadimovichMishin Aleksej ViktorovichLyapina Elizaveta AlekseevnaPopov Petr AnatolevichShevtsov Mikhail Borisovich

(73)

Holder

Federalnoe Gosudarstvennoe Avtonomnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Fiziko-Tekhnicheskij Institut Issledovatelskij Universitet)"

MODIFIED GENETIC CONSTRUCT FOR RECOMBINANT EXPRESSION AND CRYSTALLISATION OF HUMAN CYSLT1 RECEPTOR Russian patent published in 2020 - IPC C07K14/705 C12N15/62 C12N15/63

Abstract RU 2735281 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of human leukotriene receptor type 1 (human cysteinyl leikotriene receptor 1, CysLTR1), and can be used for expression of CysLT1 receptor. Disclosed is a genetic construct with a nucleotide sequence which is optimized for expression of CysLTR1 in insect cells. Also, additional truncation of disordered C-end of CysLT1 receptor prevents formation of crystals.

EFFECT: invention provides recombinant expression in a eukaryotic system on a cell surface by combining N-terminal peptide fragments and the position of partner protein b562RIL in a third intracellular loop, which also stabilizes the protein and helps to form crystalline contacts.

1 cl, 3 dwg

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RU 2 735 281 C1

Authors

Safronova Nadezhda Aleksandrovna

Luginina Aleksandra Pavlovna

Borshchevskij Valentin Ivanovich

Gusach Anastasiya Yurevna

Marin Egor Vadimovich

Mishin Aleksej Viktorovich

Lyapina Elizaveta Alekseevna

Popov Petr Anatolevich

Shevtsov Mikhail Borisovich

Dates

2020-10-29—Published

2019-11-25—Filed