FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Nucleotide sequence MITEKnn was obtained by sequencing. DNA culture used is a culture of the colistin-resistant K. pneumoniae strain obtained by Uri-select agar culturing (BioRad, USA). DNA extraction from bacterial cultures is carried out using a commercial set of DNeasy Blood & Tissue Kit reagents (Qiagen, Germany) in accordance with manufacturer's instructions. Nucleotide sequence of the DNA fragment which breaks the structure and function of the mgrB gene in a colistin-resistant strain of K. pneumoniae is described and described.
EFFECT: use of described nucleotide sequence MYTEKpnl:3GGAAGGTGCGAATAAGTCACCAAACGCAAA AAAACGCCTATGTAAGTGATTGAATTTAATT GACTAAAAGTTTCATTTTCGTTGCGG AGGGTACTTATTCGCACCTTCC-5', capable of integration and movement within the genome, as well as capable of changing the structure, function and expression of the gene, its detection in the bacterial genome will help to decipher mechanisms of bacterial resistance to antibiotics, which will facilitate rational selection of antimicrobial preparations and development of measures to overcome resistance.
1 cl, 2 dwg
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