FIELD: biotechnology.
SUBSTANCE: set of oligonucleotides for the determination of the 12FAB serotype of Streptococcus pneumoniae by real-time polymerase chain reaction has been proposed. The kit includes a forward primer, a reverse primer and a fluorescent probe represented by unique sequences. Oligonucleotides do not cross-react with other tested S. pneumoniae serotypes, amplify a region conserved for the 12FAB serotype with a specificity of 96%, and make it possible to determine the presence or absence of a serotype-specific target for 12FAB serotype S. pneumoniae in biological material samples.
EFFECT: invention provides detection of 12FAB DNA of S. pneumoniae serotype with a high degree of specificity and can be used for identification of causative agents of pneumococcal infections and evaluation of the effectiveness of immunoprophylactic measures being taken.
2 cl, 3 dwg, 1 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| OLIGONUCLEOTIDES FOR DETECTION OF 22FA SEROTYPE OF STREPTOCOCCUS PNEUMONIAE | 2021 |
|
RU2802203C2 |
| OLIGONUCLEOTIDES FOR DETECTION OF 15BC SEROTYPE OF STREPTOCOCCUS PNEUMONIAE | 2021 |
|
RU2795316C1 |
| OLIGONUCLEOTIDES FOR DETECTION OF 8 SEROTYPE OF STREPTOCOCCUS PNEUMONIAE | 2021 |
|
RU2802081C2 |
| MULTIPLEX PCR MIXTURE FOR DETERMINATION OF SEROTYPES 12FAB, 15BC, 22FA, 8 STREPTOCOCCUS PNEUMONIAE AND METHOD FOR ITS APPLICATION | 2021 |
|
RU2787181C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR DETERMINING ALLELES OF THE RS55986091 POLYMORPHISM AND METHOD FOR ITS USE | 2022 |
|
RU2804110C1 |
| OLIGONUCLEOTIDES FOR DETECTION OF SARS-COV-2 MUTATION S:Ins214EPE | 2022 |
|
RU2795017C1 |
| METHOD OF GENOTYPING TLR8 GENE USING RS3764880 POLYMORPHISM AND A SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR ITS IMPLEMENTATION | 2023 |
|
RU2805864C1 |
| METHOD OF GENOTYPING TLR2 GENE USING RS5743708 POLYMORPHISM AND A SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR ITS IMPLEMENTATION | 2023 |
|
RU2805861C1 |
| METHOD OF GENOTYPING TLR2 GENE USING RS3804100 POLYMORPHISM AND A SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR ITS IMPLEMENTATION | 2023 |
|
RU2805860C1 |
| METHOD OF GENOTYPING TLR1 GENE USING RS5743551 POLYMORPHISM AND A SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR ITS IMPLEMENTATION | 2023 |
|
RU2805859C1 |
