FIELD: biotechnology.
SUBSTANCE: invention relates to the determination of 15 VS serotype Streptococcus pneumoniae using real-time polymerase chain reaction (RTP-PCR) and can be used to identify pathogens of pneumococcal infections (PI) and evaluate the effectiveness of ongoing immunoprophylactic measures. Oligonucleotides for the determination of 15 BC of the S. pneumoniae serotype — forward primer 15BCF, reverse primer 15BCR, fluorescent probe 15BCZ, are represented by unique sequences of SEQ ID NO: 1–3 respectively.
EFFECT: at the same time, the forward 15BCF and reverse 15BCR primers are complementary to the region of the S. pneumoniae 15 BC serotype gene that does not have homology with fragments of the cps locus of representatives of other S. pneumoniae serotypes.
2 cl, 3 dwg, 1 tbl, 4 ex
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