FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry, namely to method of identifying genes of clinically significant families of β-lactamases in gram-negative microorganisms, for example, in different strains Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Burkholderia spp., Enterobacter spp., Stenotrophomonas maltophilia, Elizabethkingia meningoseptica, Serratia marcescens. Method is performed by amplification of fragments by polymerase chain reaction with set of specific oligonucleotide primers and further separation of reaction products by electrophoresis in agarose gel. With help of above method genes of following families of β-lactamase VHS are identified, CTX-M, VIM, IMP, NDM, OXA, KPC, GES, GIM and SPM.
EFFECT: present invention increases range of products for identification of families of β-lactamases VHS, CTX-M, VIM, IMP, NDM, OXA, KPC, GES, GIM and SPM.
1 cl, 4 tbl, 2 ex
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| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR ACINETOBACTER BACTERIA STRAINS AND INSOLES IDENTIFICATION AND DETERMINATION OF RESISTANCE TO BETA-LACTAM ANTIBIOTICS AND THE METHOD OF THEIR USE | 2016 |
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| METHOD FOR IDENTIFICATION OF NOSOCOMIAL OPPORTUNISTIC INFECTIONS PATHOGENS AND MARKERS OF THEIR RESISTANCE TO BETA-LACTAM ANTIBIOTICS AND GLYCOPEPTIDES IN WOMEN OF REPRODUCTIVE AGE AND NEWBORN CHILDREN FOR ANTIBACTERIAL THERAPY OPTIMISATION | 2016 |
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| METHOD FOR IDENTIFYING EXTENDED SPECTRUM CLASS A BETA-LACTAMASE GENES | 2009 |
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| OLIGONUCLEOTIDE PRIMERS FOR β LACTAMASE GENE DETECTON AND TYPING OF PATHOGENIC BURKHOLDERIA | 2011 |
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RU2474614C1 |
| DNA MICROCHIP FOR IDENTIFYING EXTENDED SPECTRUM CLASS A BETA-LACTAMASE GENES | 2009 |
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| BETA-LACTAMASE, METHOD OF ITS MODIFICATION, OBTAINING AND APPLICATION | 2011 |
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| METHOD OF GENETIC POLYMORPHISM ANALYSIS FOR CARRYING OUT POSTNATAL DNA-DIAGNOSTICS OF MUCOVISCIDOSIS | 2008 |
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RU2412247C2 |
| KLEBSIELLA PNEUMONIAE BACTERIAL STRAIN USED AS A TEST CULTURE FOR THE DETECTION OF THE NDM GENE BY POLYMERASE CHAIN REACTION FOR ACCURATE DIAGNOSIS AND PRESCRIPTION OF ANTIBACTERIAL THERAPY FOR KLEBSIELLA INFECTIONS | 2022 |
|
RU2797025C1 |
| METHOD FOR ELIMINATING FALSE-POSITIVE RESULTS CAUSED BY CONTAMINATION OF DNA POLYMERASE WITH FOREIGN DNA FRAGMENTS, WITH IDENTIFICATION OF BLATEM GENES | 2019 |
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| METHOD FOR DETECTING SPECIFIC NUCLEOTIDE SEQUENCES AND NUCLEOTIDE REPLACEMENTS BY REAL-TIME PCR WITH EFFECT OF PROBE FLUORESCENCE QUENCHING SHOWN BY PRIMER | 2010 |
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RU2451086C1 |
