FIELD: pharmaceuticals.
SUBSTANCE: invention relates to production of biologically active additives based on proteoglycans and glycosaminoglycans. Method of producing biologically active additive is characterized in that solution, containing proteoglycans and glycosaminoglycans in the form of fine suspension in 0.1 N sodium hydroxide solution at ratio of 1:200, is concentrated to the gel consistence. For this purpose, it is exposed for 20 minutes at room temperature for complete settling of aggregates and formation of stable dense sediment, then supernatant fluid is separated, sediment is centrifuged at 1000 rpm for 45 seconds and the supernatant is drained. Sedimentation, having pH 9.0–9.3, with gentle stirring is added to neutralize 0.1 N solution of hydrochloric acid, bringing solution to pH 8.0. Thereafter, the final gel residue is sublimated to solid state for at least 12 hours at −4 °C and after termination of sublimation drying are ground, conducted through a mesh filter with mesh size of 500 mcm, and the powder is packaged into solid gastro-resistant capsules. Alternatively, the final precipitate in form of concentrated gel, which ratio in total weight makes 40–55 % gel/solution—0.9 % sodium chloride, is placed into gastro-resistant capsules. Produced biologically active additive in the form of gastro-resistant capsules is orally administered.
EFFECT: invention enables to use biocompatible high-molecular glucuronic acid derivatives in pathological conditions accompanied by intoxication of various aetiology, contamination of pathogenic intestinal microflora, as well as with hepatotrophic and compensatory-reducing purpose with deficiency of these substances.
5 cl, 2 dwg, 4 tbl, 6 ex
