FIELD: medicine; pharmacology.
SUBSTANCE: urine is preliminarily cooled, kept until aggregates are formed, centrifuged to precipitate proteoglycan-free sediment, after that, precipitant, composition of which includes 4 volumes of 96° ethyl alcohol and 1 volume of 0.01 N solution of sodium hydroxide, is added to supernatant fluid in ratio 1:1 to precipitate proteoglycan, after which mixture is kept for 20 min without changing temperature to allow formation of aggregates, which are separated from supernatant fluid in mode of cooling at 0°C by centrifugation, sediment is washed by re-addition of cooled to 0°C precipitant in ratio 1:10, kept for 5 minutes until aggregates are formed, centrifuged again, supernatant fluid is separated, obtained sediment is dissolved by addition of 0.01 N of HCl solution, reducing pH value of final product to pH 7.2, remaining insufficient non-soluble sediment is separated by centrifugation, supernatant fluid, containing purified proteoglycan is used as intended.
EFFECT: method, described above, provides obtaining highly-purified proteoglycan.
3 cl, 6 dwg, 6 tbl, 3 ex
