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2 738 798

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(51)

IPC

G01N33/58(2006-01-01)

C12Q1/6806(2018-01-01)

C12Q1/6876(2018-01-01)

(21) (22)

Application

2020128826, 2020-08-31

(24)

Start date

2020-08-31

(22)

Actual filing date

2020-08-31

(45)

Published

2020-12-16

(72)

Inventor

Ashapkin Vasilii VasilevichBragina Elizaveta Efimovna

(73)

Holder

Ashapkin Vasilii VasilevichBragina Elizaveta EfimovnaKuleshov Dmitrii Nikolaevich

METHOD FOR QUANTITATIVE DETERMINATION OF VIRAL INFECTION OF SPERM CELLS Russian patent published in 2020 - IPC G01N33/58 C12Q1/6806 C12Q1/6876

Abstract RU 2738798 C1

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to virology and medical examination, and aims at quantitative determination of viral infection of spermatozoa. DNA is recovered from a sperm sample. A quantitative polymerase chain reaction is carried out with real-time registration and normalization of the obtained data with the help of NOG and BMP4 primers to obtain the average content of viral DNA on the spermatozoon. Share of infected spermatozoons is determined by electron microscopic examination of spermatozoons to obtain a percentage of infected spermatozoa. Viral load is calculated on infected sperm cell L by formula L=100×N/E.

EFFECT: invention provides simplifying and accelerating preparation of DNA samples from a sperm sample; simpler and cheaper PCR procedure; high accuracy of quantitative assessment of viral load; applicability to any DNA-containing virus; quantitative determination of viral load in all sperm cells irrespective of their type.

14 cl, 4 tbl

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RU 2 738 798 C1

Authors

Ashapkin Vasilii Vasilevich

Bragina Elizaveta Efimovna

Dates

2020-12-16—Published

2020-08-31—Filed