METHOD OF SPERMATOZOID SAMPLE PREPARATION FOR ELECTRONIC MICROSCOPY Russian patent published in 2009 - IPC G01N33/48 G01N1/28 

FIELD: medicine.

SUBSTANCE: ejaculate is dissolved at 1:10-1:20 ratio by saline, fixated by 2.5% glutaric aldehyde solution. Obtained fixated material in the form of fixation agent drop with sediment cells suspended after resuspension is applied onto aluminium foil degreased preliminarily, incubated in humid chamber at room temperature for 25÷35 minutes, the foil with attached cells is flushed by 0.1 M phosphate buffer with pH 7.2-7.4, with further fixation in 1% osmic fixation agent and dehydration. Then the foil with cells is placed to saturated uranyl acetate solution in 70% ethanol and incubated in closed test tube for 12-14 hours. Afterwards the sample is dehydrated and filled by epoxy resin, after resin polymerisation the foil is separated from obtained epoxy resin plate with spermatozoids on its surface.

EFFECT: possible examination of single spermatozoids in samples with very low concentration and obtainment of ultra thin oriented sections of spermatozoids.