METHOD FOR IDENTIFYING CANDIDA SPP_ AND OTHER YEAST-LIKE FUNGI FROM POSITIVE BLOOD CULTURE BY MATRIX LASER DESORPTION IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY (MALDI-TOF MS) IN PATIENTS WITH BLOOD FLOW INFECTION Russian patent published in 2020 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to microbiology. Disclosed is a method for identifying yeast-like fungi of the genus Candida and genus Rhodotorula, comprising sample preparation of positive blood culture and identification of yeast-like fungi by matrix-activated laser desorption ionization time-of-flight mass spectrometry. At that, sample preparation of positive blood culture is performed by means of its transfer from flask with liquid nutrient medium to a test tube with separating gel and then centrifuged at 1000 rpm for 2 minutes. Obtained supernatant is exposed to multiple sequential centrifugation and addition of deionised water. Supernatant is then removed, deionised water and 96 % ethyl alcohol are added to the sediment and mixed once more with a vortex, followed by centrifugation at 14000 rpm for 2 minutes, then supernatant is removed. Then, microcentrifuge test tube with open cover is left to complete evaporation of alcohol, followed by extraction of protein extract with formic acid and acetonitrile, the obtained supernatant is applied on a target of a mass spectrometer, after drying is coated with a matrix and identification of yeast-like fungi of the genus Candida and genus Rhodotorula is carried out until the generic identity by the value of the identification coefficient (score) from 1.1 to 1.5 and to species identity - from 1.6 and higher.

EFFECT: invention provides a wider range of technical means for accelerated identification of microorganisms from blood containing yeast-like fungi with high reliability.

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