METHOD OF DETERMINING GENUS OF BACTEREMIA AGENTS Russian patent published in 2013 - IPC C12Q1/04 G01N33/52 

Abstract RU 2495939 C2

FIELD: biotechnology.

SUBSTANCE: method comprises incubation of the blood sample in the nutritional medium, followed by detection of microbial growth in the primary hemoculture. Sample preparation of the sample under study is carried out by centrifugation. The quantitative chromatography-mass-spectrometer analysis of the sample under study is carried out with the definition of marker molecules, which are used as molecules of free and substituted higher fatty acids of cellular lipids of microorganisms. The free and substituted higher fatty acids are identified by comparing the data obtained with the database NIST of chromatography-mass-spectrometer. The genus of bacteremia agents is determined using the Table 1.

EFFECT: invention enables to provide for early diagnosis of bacteremia.

5 dwg, 5 ex

Similar patents RU2495939C2

Title Year Author Number
METHOD OF DETECTION OF PATHOGEN OF INFECTIOUS PROCESS IN STERILE BIOLOGICAL MEDIA OF MICROORGANISM 1997
  • Osipov G.A.
  • Beloborodova N.V.
RU2146368C1
CALIBRATION METHOD OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) SYSTEM EQUIPPED WITH SPECIAL SOFTWARE FOR DETERMINATION OF MARKERS OF MICROORGANISMS IN TESTED SAMPLE OF MATERIAL OF BIOLOGICAL ORIGIN 2012
  • Osipov Georgij Andreevich
RU2501011C2
LABORATORY DIAGNOSTIC TECHNIQUE FOR SEPSIS 2009
  • Beloborodova Natal'Ja Vladimirovna
  • Khodakova Anastasija Sergeevna
  • Olenin Andrej Jur'Evich
RU2423704C1
METHOD OF DETERMINATION OF GENUS (SPECIES) COMPOSITION OF MICROORGANISM ASSOCIATION 1993
  • Osipov Georgij Andreevich
RU2086642C1
SAMPLE PREPARATION METHOD FOR ACCELERATED IDENTIFICATION OF MICROORGANISMS FROM POSITIVE HEMATOLOGICAL CULTURES 2021
  • Khaliulin Almaz Vadimovich
  • Lyamin Artem Viktorovich
  • Gusyakova Oksana Anatolevna
  • Kozlov Andrej Vladimirovich
  • Baldina Olga Anatolevna
RU2766185C1
METHOD FOR IDENTIFYING CANDIDA SPP_ AND OTHER YEAST-LIKE FUNGI FROM POSITIVE BLOOD CULTURE BY MATRIX LASER DESORPTION IONIZATION TIME-OF-FLIGHT MASS SPECTROMETRY (MALDI-TOF MS) IN PATIENTS WITH BLOOD FLOW INFECTION 2020
  • Klyasova Galina Aleksandrovna
  • Malchikova Anna Olegovna
  • Dzhulakyan Unan Levonovich
RU2739758C1
METHOD FOR DETERMINING REFERENCE VALUES OF INDICATORS OF MICROORGANISMS IN THE PROSTATE GLAND, INVESTIGATED USING CHROMATO-MASS SPECTROMETRY METHOD 2022
  • Kadyrov Zieratsho Abdulloevich
  • Stepanov Vladimir Sergeevich
  • Bystrova Olga Vitalevna
  • Ramishvili Shota Vladimirovich
  • Aldyrakov Emilbek Mamasabyrovich
RU2791489C1
METHOD FOR IDENTIFYING ETHYL GLUCURONIDE IN BLOOD 2020
  • Savchuk Sergej Aleksandrovich
  • Novikov Andrej Petrovich
  • Rizvanova Liliya Nazhipovna
  • Buryak Aleksej Konstantinovich
  • Shaborshin Nikolaj Yurevich
RU2750408C1
METHOD TO REVEAL AND DETERMINE ORIGIN OF UNKNOWN SUBSTANCES IN ALCOHOLIC DRINKS 2009
  • Savchuk Sergej Aleksandrovich
  • Chibisova Margarita Vjacheslavovna
  • Anokhin Leonid Andreevich
RU2392616C1
METHOD FOR DETERMINING REFERENCE VALUES OF MICROORGANISM INDICATORS ANALYZED BY CHROMATO-MASS SPECTROMETRY 2019
  • Tokarev Mikhail Iurevich
  • Platonova Anna Gennadevna
RU2715223C1

RU 2 495 939 C2

Authors

Popov Dmitrij Aleksandrovich

Ovseenko Svetlana Timofeevna

Osipov Georgij Andreevich

Vostrikova Tat'Jana Jur'Evna

Dates

2013-10-20Published

2011-08-25Filed