FIELD: biotechnology.
SUBSTANCE: method comprises incubation of the blood sample in the nutritional medium, followed by detection of microbial growth in the primary hemoculture. Sample preparation of the sample under study is carried out by centrifugation. The quantitative chromatography-mass-spectrometer analysis of the sample under study is carried out with the definition of marker molecules, which are used as molecules of free and substituted higher fatty acids of cellular lipids of microorganisms. The free and substituted higher fatty acids are identified by comparing the data obtained with the database NIST of chromatography-mass-spectrometer. The genus of bacteremia agents is determined using the Table 1.
EFFECT: invention enables to provide for early diagnosis of bacteremia.
5 dwg, 5 ex
Authors
Dates
2013-10-20—Published
2011-08-25—Filed