METHOD OF LABORATORY DIAGNOSTICS OF MALE REPRODUCTIVE FUNCTION Russian patent published in 2023 - IPC G01N33/68 G01N33/487 G01N1/28 G01N33/569 C12Q1/06 C12Q1/68 

FIELD: medicine; urology; andrology.

SUBSTANCE: invention can be used for laboratory diagnostics of male reproductive dysfunction. Biological material is collected. Based on the data obtained, a conclusion is made about the male reproductive function. Ejaculate is used as biological material. For diagnosis, microbiological and molecular analysis are carried out simultaneously. Microbiological analysis, namely the identification of the obtained colonies of microorganisms is carried out by quantitative measurement of the concentration of microorganisms of the resident flora or its microbial associations and by measuring the number of non-resident bacteria in concentration ≥10³CFU/ml using time-of-flight matrix-associated laser desorption ionization mass spectrometry or on a microbiological analyzer. Molecular analysis, namely the identification and quantitative analysis of the ejaculate sediment biotope according to the spectrum of opportunistic microorganisms, is carried out on detecting amplifiers, which includes the following: gram-positive facultative anaerobic microorganisms Streptococcus spp., Staphylococcus spp., Corynebacterium spp., Gram-negative facultative anaerobic microorganisms Haemophil us spp., Pseudomonas aeruginosa/Ralstonia spp./Burkholderia spp., group Enterobacteriaceae/Enterococcus spp., obligate anaerobic opportunistic pathogens — Eubacterium spp., Sneathia spp./Leptotrichia spp./Fusobacterium spp., Megasphaera spp./Veillonella s pp./Dialister spp., Bacteroides spp./Porphyromonas spp./Prevotella spp., Anaerococcus spp., Peptostreptococcus spp., Atopobium cluster, and fungi of Candida genus. Violation of male reproductive function is diagnosed when the concentration of DNA in the sediment of the ejaculate is more than or equal to 10³ GE/ml: obligate anaerobic opportunistic pathogens and/or gram-negative facultative anaerobic microorganisms and/or Enterobacteriaceae/Enterococcus spp. and/or mycoplasmas and/or fungi of the genus Candida, if microorganisms of the resident flora or its microbial associations or non-resident bacteria are found in the sediment of the ejaculate at a concentration of more than or equal to 10³ CFU/ml.

EFFECT: method provides the possibility of expanding the range of diagnostics of etiological factors of an infectious nature of a violation of male reproductive function, increasing the information content and reliability of diagnostics due to the simultaneous microbiological and molecular analysis of the ejaculate sediment.

4 cl, 17 dwg, 3 ex