CARRIER CONTAINING A DRUG INTO A CELL FOR FORMING AN IMMUNE COMPLEX Russian patent published in 2020 - IPC C12N15/09 G01N33/50 

FIELD: biochemistry.

SUBSTANCE: invention refers to using an antibody containing a human IgG Fc region and two antigen-binding domains to eliminate plasma antigens. Also disclosed is a method of screening an antibody having a function of eliminating an antigen from plasma, involving contacting the antigen with an antigen-binding domain at pH 5.8 and pH 7.4 respectively, obtaining an antigen-binding domain whose antigen-binding activity varies depending on pH conditions and has a KD (pH 5.8)/KD (pH 7.4) value, defined as the KD ratio for the given antigen at pH 5.8 and KD for the given antigen at pH 7.4, equal to 2 or more. Invention also relates to a method of producing an antibody having a function of eliminating an antigen from plasma, comprising obtaining an antigen-binding domain, whose antigen-binding activity varies depending on the pH conditions, and has a KD (pH 5.8)/KD (pH 7.4) value, defined as the KD ratio for the given antigen at pH 5.8 and KD for the given antigen at pH 7.4, equal to 2 or more.

EFFECT: invention enables to efficiently produce an antibody possessing the antigen elimination function.

23 cl, 27 dwg, 16 tbl, 16 ex