METHOD FOR DIFFERENTIAL DIAGNOSIS OF HIGH GRADE GLIOMAS Russian patent published in 2022 - IPC C12N1/00 C12Q1/68 

FIELD: molecular biology.

SUBSTANCE: invention relates to the field of molecular biology and can be used for the differential diagnosis of brain glial tumors of high grades of malignancy. A method for the differential diagnosis of high-grade gliomas is described, in which total RNA is isolated from a glioma tissue sample, the synthesis of the 1st strand of cDNA is carried out using a reverse transcription reaction, real-time PCR is performed with primers matched to the mRNA of the TEK, C3, MKI67, HIF1A genes, OLIG2, TNFRSF1A. The degree of malignancy of glioma "y" is calculated by the formula: у=1,69-2,844×X₁-0.883×X₂-0.043×X₃+0.081×X₄+0.169×X₅-0.04×X₆, where X₁ - TEK gene expression; X₂ - expression of the C3 gene; X₃ - MKI67 gene expression; X₄ - HIF1A gene expression; X₅ - expression of the OLIG2 gene; X₆ - TNFRSF1A gene expression. If the values ​​of "y" are greater than 2.4, but less than 3.4, the tumor is classified as Grade III; with "y" greater than 3.4, the diagnosis is "glioblastoma", Grade IV.

EFFECT: increasing the accuracy of the method, reducing labor intensity and simplifying it.

1 cl, 1 dwg, 1 ex