METHOD FOR DIFFERENTIAL DIAGNOSIS OF GLIOMAS BASED ON ANALYSIS OF GENE EXPRESSION AND MICRO-RNA Russian patent published in 2019 - IPC C12N15/00 C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and medicine. Disclosed is a method for differential diagnosis of gliomas based on analysis of gene expression and micro-RNA, involving extraction of total RNA from tissue samples by gliomas and perifocal zone, reverse transcription, with further RT-PCR real-time amplification, characterized by using high-specific primers for genetic loci EGFR, MSI1, PSMC4, TBP, RPLO, and micro-RNA hsa-miR-92-1-5p, hsa-miR-126-5p, hsa-miR-7-5p, obtained data are analyzed and coefficient of relative E expression is calculated using three highly specific reference genes: PSMC4, TBP, RPLO and two reference highly specific micro-RNAs: hsa-miR-126-5p, hsa-miR-7-5p, obtained values of E in tumor tissue are compared with reference values and values of E_EGFR<0.5 or E_EGFR> 1.5, E_MSI1<0.5 or E_MSI1>1.5, E_{hsa-miR-92a-1-5p}<0.5 or E_{hsa-miR-92a-1-5p}>1.5, at least one loci of the patient is diagnosed with high-grade glioma III, IV; absence of changes of expression of specified loci relative to control testifies to conformity of tumor of II degree of malignancy.

EFFECT: invention enables efficient differentiation of glioma in medicine.

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